Inoculation means applying spores or spawn in what will be used as substrate or nutrient media.

During inoculation it’s extremely important to work in a sterilized environment. During this stage the spores and the substrate are very vulnerable to contamination.

You can inoculate the substrate with:

  • Spore prints
  • Spore syringes
  • Mycelium on agar
  • Live tissue
  • Spawn